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A dual protease approach for expression and affinity purification of recombinant proteins
We describe a new method for affinity purification of recombinant proteins using a dual protease protocol. Escherichia coli maltose binding protein (MBP) is employed as an N-terminal tag to increase the yield and solubility of its fusion partners. The MBP moiety is then removed by rhinovirus 3C prot...
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| Pubblicato in: | Anal Biochem |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4877217/ https://ncbi.nlm.nih.gov/pubmed/27105777 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2016.04.006 |
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