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Positional effects of fusion partners on the yield and solubility of MBP fusion proteins

Escherichia coli maltose-binding protein (MBP) is exceptionally effective at promoting the solubility of its fusion partners. However, there are conflicting reports in the literature claiming that 1) MBP is an effective solubility enhancer only when it is joined to the N-terminus of an aggregation-p...

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Detalhes bibliográficos
Publicado no:Protein Expr Purif
Main Authors: Raran-Kurussi, Sreejith, Keefe, Karina, Waugh, David S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4393804/
https://ncbi.nlm.nih.gov/pubmed/25782741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2015.03.004
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