PRODUCTION OF RECOMBINANT, NON-TAGGED PHENYLALANINE AMMONIA-LYASES EMPLOYING TEV PROTEASE-REMOVABLE AFFINITY TAGS

Nowadays, protein purification by the aid of affinity tags can be carried out with high speed and efficiency. However, in several cases, affinity tags can significantly alter the key properties of enzymes, especially activity and/or thermostability. This study focused on the purification of the n...

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Bibliografske podrobnosti

Principais autores:	Alina FILIP, Zsófia BATA, Anca Elena ANGHEL, László POPPE, László Csaba BENCZE
Format:	Artigo
Jezik:	Inglês
Izdano:	Babes-Bolyai University 2022-12-01
Serija:	Studia Universitatis Babes-Bolyai Chemia
Teme:	affinity tags, phenylalanine ammonia-lyase, TEV (Tobacco Etch Virus) protease, thermostability, specific enzyme activity, oligomerization state.
Online dostop:	https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/7742
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