PRODUCTION OF RECOMBINANT, NON-TAGGED PHENYLALANINE AMMONIA-LYASES EMPLOYING TEV PROTEASE-REMOVABLE AFFINITY TAGS

Nowadays, protein purification by the aid of affinity tags can be carried out with high speed and efficiency. However, in several cases, affinity tags can significantly alter the key properties of enzymes, especially activity and/or thermostability. This study focused on the purification of the n...

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Autors principals:	Alina FILIP, Zsófia BATA, Anca Elena ANGHEL, László POPPE, László Csaba BENCZE
Format:	Artigo
Idioma:	Inglês
Publicat:	Babes-Bolyai University 2022-12-01
Col·lecció:	Studia Universitatis Babes-Bolyai Chemia
Matèries:	affinity tags, phenylalanine ammonia-lyase, TEV (Tobacco Etch Virus) protease, thermostability, specific enzyme activity, oligomerization state.
Accés en línia:	https://studia.reviste.ubbcluj.ro/index.php/chemia/article/view/7742
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