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Differential Temperature Dependence of Tobacco Etch Virus and Rhinovirus 3C Proteases
Because of their stringent sequence specificity, the 3C-like proteases from tobacco etch virus (TEV()) and human rhinovirus are often used for the removal of affinity tags. The latter enzyme is rumored to have greater catalytic activity at 4°C, the temperature at which fusion protein substrates are...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4196241/ https://ncbi.nlm.nih.gov/pubmed/23395976 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.01.031 |
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