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RNA polymerase clamp conformational dynamics: long-lived states and modulation by crowding, cations, and nonspecific DNA binding

The RNA polymerase (RNAP) clamp, a mobile structural element conserved in RNAP from all domains of life, has been proposed to play critical roles at different stages of transcription. In previous work, we demonstrated using single-molecule Förster resonance energy transfer (smFRET) that RNAP clamp i...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Mazumder, Abhishek, Wang, Anna, Uhm, Heesoo, Ebright, Richard H, Kapanidis, Achillefs N
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2021
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7969002/
https://ncbi.nlm.nih.gov/pubmed/33589919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab074
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