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CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells
Precise and efficient genome modifications provide powerful tools for biological studies. Previous CRISPR gene knockout methods in cell lines have relied on frameshifts caused by stochastic insertion/deletion in all alleles. However, this method is inefficient for genes with high copy number due to...
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| Veröffentlicht in: | Nat Protoc |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7961850/ https://ncbi.nlm.nih.gov/pubmed/32958931 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-0383-8 |
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