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Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells
Genome-wide screens in Drosophila cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded pooled screens. Here, we developed a site-specific integration strategy for l...
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| Pubblicato in: | eLife |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
eLife Sciences Publications, Ltd
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6063728/ https://ncbi.nlm.nih.gov/pubmed/30051818 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.36333 |
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