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Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence

The precise spatial localization of single molecules in three dimensions is an important basis for single molecule localization microscopy (SMLM) and tracking. At distances up to a few hundred nanometers from the coverslip, evanescent wave coupling into the glass, also known as supercritical angle f...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Zelger, Philipp, Bodner, Lisa, Offterdinger, Martin, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7901312/
https://ncbi.nlm.nih.gov/pubmed/33680543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.413018
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