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Supercritical Angle Fluorescence Correlation Spectroscopy

We explore the potential of a supercritical angle (SA) objective for fluorescence correlation spectroscopy (FCS). This novel microscope objective combines tight focusing by an aspheric lens with strong axial confinement of supercritical angle fluorescence collection by a parabolic mirror lens, resul...

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Autori principali: Ries, Jonas, Ruckstuhl, Thomas, Verdes, Dorinel, Schwille, Petra
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Biophysical Society 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2134864/
https://ncbi.nlm.nih.gov/pubmed/17827221
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.115998
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