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Precise Measurement of Diffusion Coefficients using Scanning Fluorescence Correlation Spectroscopy

We have implemented scanning fluorescence correlation spectroscopy (sFCS) for precise determination of diffusion coefficients of fluorescent molecules in solution. The measurement volume where the molecules are excited, and from which the fluorescence is detected, was scanned in a circle with radius...

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Autores principales: Petrášek, Zdeněk, Schwille, Petra
Formato: Artigo
Lenguaje:Inglês
Publicado: The Biophysical Society 2008
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2212689/
https://ncbi.nlm.nih.gov/pubmed/17933881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.108811
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