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Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence

The precise spatial localization of single molecules in three dimensions is an important basis for single molecule localization microscopy (SMLM) and tracking. At distances up to a few hundred nanometers from the coverslip, evanescent wave coupling into the glass, also known as supercritical angle f...

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Bibliographische Detailangaben
Veröffentlicht in:Biomed Opt Express
Hauptverfasser: Zelger, Philipp, Bodner, Lisa, Offterdinger, Martin, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander
Format: Artigo
Sprache:Inglês
Veröffentlicht: Optical Society of America 2021
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7901312/
https://ncbi.nlm.nih.gov/pubmed/33680543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.413018
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