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Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy

Single molecule localization microscopy (SMLM) is one of the key techniques that break the classical resolution limit in optical imaging. It is based on taking multiple recordings of a sample, each showing only a sparse arrangement of spatially well separated fluorescent molecules which can be local...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Zelger, Philipp, Bodner, Lisa, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7041438/
https://ncbi.nlm.nih.gov/pubmed/32206395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.375678
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