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Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy

Single molecule localization microscopy (SMLM) is one of the key techniques that break the classical resolution limit in optical imaging. It is based on taking multiple recordings of a sample, each showing only a sparse arrangement of spatially well separated fluorescent molecules which can be local...

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Vydáno v:Biomed Opt Express
Hlavní autoři: Zelger, Philipp, Bodner, Lisa, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander
Médium: Artigo
Jazyk:Inglês
Vydáno: Optical Society of America 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7041438/
https://ncbi.nlm.nih.gov/pubmed/32206395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.375678
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