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Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy

Single molecule localization microscopy (SMLM) is one of the key techniques that break the classical resolution limit in optical imaging. It is based on taking multiple recordings of a sample, each showing only a sparse arrangement of spatially well separated fluorescent molecules which can be local...

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Détails bibliographiques
Publié dans:Biomed Opt Express
Auteurs principaux: Zelger, Philipp, Bodner, Lisa, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander
Format: Artigo
Langue:Inglês
Publié: Optical Society of America 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7041438/
https://ncbi.nlm.nih.gov/pubmed/32206395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.375678
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