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Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence

The precise spatial localization of single molecules in three dimensions is an important basis for single molecule localization microscopy (SMLM) and tracking. At distances up to a few hundred nanometers from the coverslip, evanescent wave coupling into the glass, also known as supercritical angle f...

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Vydáno v:Biomed Opt Express
Hlavní autoři: Zelger, Philipp, Bodner, Lisa, Offterdinger, Martin, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander
Médium: Artigo
Jazyk:Inglês
Vydáno: Optical Society of America 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7901312/
https://ncbi.nlm.nih.gov/pubmed/33680543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.413018
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