Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato

DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency up to 72%...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Zhao, Xue, Jayarathna, Shishanthi, Turesson, Helle, Fält, Ann-Sofie, Nestor, Gustav, González, Matías N., Olsson, Niklas, Beganovic, Mirela, Hofvander, Per, Andersson, Roger, Andersson, Mariette
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2021
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7900246/
https://ncbi.nlm.nih.gov/pubmed/33619312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-83462-z
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