Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

KEY MESSAGE: Altered starch quality with full knockout of GBSS gene function in potato was achieved using CRISPR-Cas9 technology, through transient transfection and regeneration from isolated protoplasts. ABSTRACT: Site-directed mutagenesis (SDM) has shown great progress in introducing precisely tar...

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Bibliografski detalji
Izdano u:Plant Cell Rep
Glavni autori: Andersson, Mariette, Turesson, Helle, Nicolia, Alessandro, Fält, Ann-Sofie, Samuelsson, Mathias, Hofvander, Per
Format: Artigo
Jezik:Inglês
Izdano: Springer Berlin Heidelberg 2016
Teme:
Original Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5206254/
https://ncbi.nlm.nih.gov/pubmed/27699473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00299-016-2062-3
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