Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato

DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency up to 72%...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Zhao, Xue, Jayarathna, Shishanthi, Turesson, Helle, Fält, Ann-Sofie, Nestor, Gustav, González, Matías N., Olsson, Niklas, Beganovic, Mirela, Hofvander, Per, Andersson, Roger, Andersson, Mariette
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7900246/
https://ncbi.nlm.nih.gov/pubmed/33619312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-83462-z
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