Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato

DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency up to 72%...

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Published in:Sci Rep
Main Authors: Zhao, Xue, Jayarathna, Shishanthi, Turesson, Helle, Fält, Ann-Sofie, Nestor, Gustav, González, Matías N., Olsson, Niklas, Beganovic, Mirela, Hofvander, Per, Andersson, Roger, Andersson, Mariette
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2021
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7900246/
https://ncbi.nlm.nih.gov/pubmed/33619312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-83462-z
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