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Generation of Sex-Reversed Female Clonal Mice via CRISPR-Cas9-Mediated Y Chromosome Deletion in Male Embryonic Stem Cells

Mice derived entirely from embryonic stem (ES) cells can be generated through tetraploid complementation. Although XY male ES cell lines are commonly used in this system, occasionally, monosomic XO female mice are produced through spontaneous Y chromosome loss. Here, we describe an efficient method...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:CRISPR J
Prif Awduron: Qin, Yiren, Wong, Bokey, Zhong, Liangwen, Geng, Fuqiang, Parada, Luis F., Wen, Duancheng
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Mary Ann Liebert, Inc., publishers 2021
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7898403/
https://ncbi.nlm.nih.gov/pubmed/33567216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2020.0074
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