One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes

BACKGROUND: The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult to achieve targeted gene knock-ins. The homology-mediated end joining (HMEJ)-based strategy has been shown to increase knock-in efficiency relative to HR,...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Owen, Joseph R., Hennig, Sadie L., McNabb, Bret R., Mansour, Tamer A., Smith, Justin M., Lin, Jason C., Young, Amy E., Trott, Josephine F., Murray, James D., Delany, Mary E., Ross, Pablo J., Van Eenennaam, Alison L.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2021
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7881600/
https://ncbi.nlm.nih.gov/pubmed/33581720
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-021-07418-3
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