Nondestructive enzymatic deamination enables single-molecule long-read amplicon sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution

The predominant methodology for DNA methylation analysis relies on the chemical deamination by sodium bisulfite of unmodified cytosine to uracil to permit the differential readout of methylated cytosines. Bisulfite treatment damages the DNA, leading to fragmentation and loss of long-range methylatio...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
में प्रकाशित:Genome Res
मुख्य लेखकों: Sun, Zhiyi, Vaisvila, Romualdas, Hussong, Laura-Madison, Yan, Bo, Baum, Chloé, Saleh, Lana, Samaranayake, Mala, Guan, Shengxi, Dai, Nan, Corrêa, Ivan R., Pradhan, Sriharsa, Davis, Theodore B., Evans, Thomas C., Ettwiller, Laurence M.
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: Cold Spring Harbor Laboratory Press 2021
विषय:
Method
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC7849414/
https://ncbi.nlm.nih.gov/pubmed/33468551
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.265306.120
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