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Single base resolution analysis of 5-methylcytosine and 5-hydroxymethylcytosine by RRBS and TAB-RRBS

Sodium bisulfite-assisted deamination of cytosine forms the basis for conducting single base resolution analysis of 5-methylcytosine in DNA. The TET family of proteins represents a group of enzymes that can oxidize 5-methylcytosine to 5-hydroxymethylcytosine. A modification of the bisulfite-based DN...

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Publicado en:Methods Mol Biol
Autores principales: Hahn, Maria A., Li, Arthur X., Wu, Xiwei, Pfeifer, Gerd P.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4340590/
https://ncbi.nlm.nih.gov/pubmed/25421665
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1804-1_14
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