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Catalytic Mechanism of Non-Target DNA Cleavage in CRISPR-Cas9 Revealed by Ab Initio Molecular Dynamics

CRISPR-Cas9 is a cutting-edge genome editing technology, which uses the endonuclease Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is promising to treat a myriad of human genetic diseases. Nevertheless, the molecular basis of DNA cleavage, which is a fundamental...

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Detalhes bibliográficos
Publicado no:ACS Catal
Main Authors: Casalino, Lorenzo, Nierzwicki, Łukasz, Jinek, Martin, Palermo, Giulia
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842700/
https://ncbi.nlm.nih.gov/pubmed/33520346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acscatal.0c03566
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