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CRISPR-Cas9 conformational activation as elucidated from enhanced molecular simulations

CRISPR-Cas9 has become a facile genome editing technology, yet the structural and mechanistic features underlying its function are unclear. Here, we perform extensive molecular simulations in an enhanced sampling regime, using a Gaussian-accelerated molecular dynamics (GaMD) methodology, which probe...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Palermo, Giulia, Miao, Yinglong, Walker, Ross C., Jinek, Martin, McCammon, J. Andrew
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5514767/
https://ncbi.nlm.nih.gov/pubmed/28652374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1707645114
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