Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus

The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA throu...

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Dades bibliogràfiques
Publicat a:Bio Protoc
Autors principals: Neuhausser, Werner M., Oh, Hyung S., Eggan, Pierce, Angelova, Magdalena, Kirchner, Rory, Eggan, Kevin C., Knipe, David M.
Format: Artigo
Idioma:Inglês
Publicat: Bio-Protocol 2020
Matèries:
Methods Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842287/
https://ncbi.nlm.nih.gov/pubmed/33659408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3748
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