Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus

The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA throu...

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Foilsithe in:Bio Protoc
Main Authors: Neuhausser, Werner M., Oh, Hyung S., Eggan, Pierce, Angelova, Magdalena, Kirchner, Rory, Eggan, Kevin C., Knipe, David M.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Bio-Protocol 2020
Ábhair:
Methods Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842287/
https://ncbi.nlm.nih.gov/pubmed/33659408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3748
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