In‐Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells

We report here the in‐cell NMR‐spectroscopic observation of the binding of the cognate ligand 2′‐deoxyguanosine to the aptamer domain of the bacterial 2′‐deoxyguanosine‐sensing riboswitch in eukaryotic cells, namely Xenopus laevis oocytes and in human HeLa cells. The riboswitch is sufficiently stabl...

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Bibliographic Details
Published in:Angew Chem Int Ed Engl
Main Authors: Broft, P., Dzatko, S., Krafcikova, M., Wacker, A., Hänsel‐Hertsch, Robert, Dötsch, Volker, Trantirek, L., Schwalbe, Harald
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2020
Subjects:
Research Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7839747/
https://ncbi.nlm.nih.gov/pubmed/32975353
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.202007184
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