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Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

BACKGROUND: Pichia pastoris (Komagataella phaffii) is an important platform for heterologous protein production due to its growth to high cell density and outstanding secretory capabilities. Recent developments in synthetic biology have extended the toolbox for genetic engineering of P. pastoris to...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna-Flores, Carlos, Chen, Xiaojing, von Hellens, Juhani, Speight, Robert
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7836175/
https://ncbi.nlm.nih.gov/pubmed/33494776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01499-7
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