Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli

The phytase gene appA(S) was isolated from Shigella sp. CD2 genomic library. The 3.8 kb DNA fragment contained 1299 bp open reading frame encoding 432 amino acid protein (AppA(S)) with 22 amino acid signal peptide at N-terminal and three sites of N-glycosylation. AppA(S) contained the active site RH...

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Библиографические подробности
Опубликовано в: :PLoS One
Главные авторы: Pal Roy, Moushree, Mazumdar, Deepika, Dutta, Subhabrata, Saha, Shyama Prasad, Ghosh, Shilpi
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2016
Предметы:
Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4726635/
https://ncbi.nlm.nih.gov/pubmed/26808559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0145745
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