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Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli

The phytase gene appA(S) was isolated from Shigella sp. CD2 genomic library. The 3.8 kb DNA fragment contained 1299 bp open reading frame encoding 432 amino acid protein (AppA(S)) with 22 amino acid signal peptide at N-terminal and three sites of N-glycosylation. AppA(S) contained the active site RH...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Pal Roy, Moushree, Mazumdar, Deepika, Dutta, Subhabrata, Saha, Shyama Prasad, Ghosh, Shilpi
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4726635/
https://ncbi.nlm.nih.gov/pubmed/26808559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0145745
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