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Expression of a Bacillus Phytase C Gene in Pichia pastoris and Properties of the Recombinant Enzyme

The cloning and expression of a native gene encoding a Bacillus subtilis phytase using Pichia pastoris as the host is described. In addition, the influence of N-glycosylation on the biochemical properties of the B. subtilis phytase, the influence of pH on the thermostability of the recombinant and n...

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Detalhes bibliográficos
Main Authors: Guerrero-Olazarán, Martha, Rodríguez-Blanco, Lilí, Carreon-Treviño, J. Gerardo, Gallegos-López, Juan A., Viader-Salvadó, José M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2918954/
https://ncbi.nlm.nih.gov/pubmed/20601512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00762-10
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