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Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris
BACKGROUND: Pichia pastoris (Komagataella phaffii) is an important platform for heterologous protein production due to its growth to high cell density and outstanding secretory capabilities. Recent developments in synthetic biology have extended the toolbox for genetic engineering of P. pastoris to...
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| 出版年: | Microb Cell Fact |
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| 主要な著者: | , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
BioMed Central
2021
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7836175/ https://ncbi.nlm.nih.gov/pubmed/33494776 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01499-7 |
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