Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

BACKGROUND: Pichia pastoris (Komagataella phaffii) is an important platform for heterologous protein production due to its growth to high cell density and outstanding secretory capabilities. Recent developments in synthetic biology have extended the toolbox for genetic engineering of P. pastoris to...

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Vydáno v:Microb Cell Fact
Hlavní autoři: Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna-Flores, Carlos, Chen, Xiaojing, von Hellens, Juhani, Speight, Robert
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2021
Témata:
Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7836175/
https://ncbi.nlm.nih.gov/pubmed/33494776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01499-7
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