Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

BACKGROUND: Phytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme development has focused on increasing thermostability to withstand the high temperatures during industrial steam pelleting. Increasing thermostability o...

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Bibliografiske detaljer
Udgivet i:Biotechnol Biofuels
Main Authors: Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna-Flores, Carlos H., Chen, Xiaojing, von Hellens, Juhani, Mahler, Stephen, Speight, Robert
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2021
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8010977/
https://ncbi.nlm.nih.gov/pubmed/33789740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13068-021-01936-8
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