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Directed Evolution of the Methanosarcina barkeri Pyrrolysyl tRNA/aminoacyl tRNA Synthetase Pair for Rapid Evaluation of Sense Codon Reassignment Potential

Genetic code expansion has largely focused on the reassignment of amber stop codons to insert single copies of non-canonical amino acids (ncAAs) into proteins. Increasing effort has been directed at employing the set of aminoacyl tRNA synthetase (aaRS) variants previously evolved for amber suppressi...

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Bibliografiske detaljer
Udgivet i:Int J Mol Sci
Main Authors: Schwark, David G., Schmitt, Margaret A., Fisk, John D.
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7830368/
https://ncbi.nlm.nih.gov/pubmed/33477414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms22020895
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