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Precise and broad scope genome editing based on high-specificity Cas9 nickases
RNA-guided nucleases (RGNs) based on CRISPR systems permit installing short and large edits within eukaryotic genomes. However, precise genome editing is often hindered due to nuclease off-target activities and the multiple-copy character of the vast majority of chromosomal sequences. Dual nicking R...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7826261/ https://ncbi.nlm.nih.gov/pubmed/33398349 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1236 |
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