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Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs
Cas9 nucleases permit rapid and efficient generation of gene-edited cell lines. However, in typical protocols, mutations are intentionally introduced into the donor template to avoid the cleavage of donor template or re-cleavage of the successfully edited allele, compromising the fidelity of the iso...
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| Publicado no: | Sci Rep |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8110799/ https://ncbi.nlm.nih.gov/pubmed/33972655 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-89312-2 |
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