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Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs

Cas9 nucleases permit rapid and efficient generation of gene-edited cell lines. However, in typical protocols, mutations are intentionally introduced into the donor template to avoid the cleavage of donor template or re-cleavage of the successfully edited allele, compromising the fidelity of the iso...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Sci Rep
Prif Awduron: Li, Pan P., Margolis, Russell L.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Nature Publishing Group UK 2021
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC8110799/
https://ncbi.nlm.nih.gov/pubmed/33972655
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-89312-2
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