Multiplexed CRISPR/CAS9‐mediated engineering of pre‐clinical mouse models bearing native human B cell receptors

B‐cell receptor (BCR) knock‐in (KI) mouse models play an important role in vaccine development and fundamental immunological studies. However, the time required to generate them poses a bottleneck. Here we report a one‐step CRISPR/Cas9 KI methodology to combine the insertion of human germline immuno...

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Vydáno v:EMBO J
Hlavní autoři: Wang, Xuesong, Ray, Rashmi, Kratochvil, Sven, Melzi, Eleonora, Lin, Ying‐Cing, Giguere, Sophie, Xu, Liling, Warner, John, Cheon, Diane, Liguori, Alessia, Groschel, Bettina, Phelps, Nicole, Adachi, Yumiko, Tingle, Ryan, Wu, Lin, Crotty, Shane, Kirsch, Kathrin H, Nair, Usha, Schief, William R, Batista, Facundo D
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2020
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7809789/
https://ncbi.nlm.nih.gov/pubmed/33258500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.2020105926
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