One‐step CRISPR/Cas9 method for the rapid generation of human antibody heavy chain knock‐in mice

Here, we describe a one‐step, in vivo CRISPR/Cas9 nuclease‐mediated strategy to generate knock‐in mice. We produced knock‐in (KI) mice wherein a 1.9‐kb DNA fragment bearing a pre‐arranged human B‐cell receptor heavy chain was recombined into the native murine immunoglobulin locus. Our methodology re...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Lin, Ying‐Cing, Pecetta, Simone, Steichen, Jon M, Kratochvil, Sven, Melzi, Eleonora, Arnold, Johan, Dougan, Stephanie K, Wu, Lin, Kirsch, Kathrin H, Nair, Usha, Schief, William R, Batista, Facundo D
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6138433/
https://ncbi.nlm.nih.gov/pubmed/30087111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.201899243
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