Engineered dual selection for directed evolution of SpCas9 PAM specificity

The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition to base-pairing interactions between its guide RNA and target DNA. Previous reports have established that the PAM...

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Bibliographic Details
Published in:Nat Commun
Main Authors: Goldberg, Gregory W., Spencer, Jeffrey M., Giganti, David O., Camellato, Brendan R., Agmon, Neta, Ichikawa, David M., Boeke, Jef D., Noyes, Marcus B.
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2021
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7807044/
https://ncbi.nlm.nih.gov/pubmed/33441553
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-20650-x
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