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Engineered dual selection for directed evolution of SpCas9 PAM specificity
The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition to base-pairing interactions between its guide RNA and target DNA. Previous reports have established that the PAM...
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| Foilsithe in: | Nat Commun |
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| Main Authors: | , , , , , , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
Nature Publishing Group UK
2021
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7807044/ https://ncbi.nlm.nih.gov/pubmed/33441553 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-20650-x |
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