enAsCas12a Enables CRISPR-Directed Evolution to Screen for Functional Drug Resistance Mutations in Sequences Inaccessible to SpCas9

While drug resistance mutations provide the gold standard proof for drug target engagement, target deconvolution of inhibitors identified from a phenotypic screen remains challenging. Genetic screening for functional in-frame drug resistance mutations by tiling CRISPR-Cas nucleases across protein co...

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Pubblicato in:Mol Ther
Autori principali: Neggers, Jasper Edgar, Jacquemyn, Maarten, Dierckx, Tim, Kleinstiver, Benjamin Peter, Thibaut, Hendrik Jan, Daelemans, Dirk
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society of Gene & Cell Therapy 2021
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Original Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7791016/
https://ncbi.nlm.nih.gov/pubmed/33002419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymthe.2020.09.025
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