Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens

The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used to systematically create gene knockouts in a varie...

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Библиографические подробности
Опубликовано в: :G3 (Bethesda)
Главные авторы: Hart, Traver, Tong, Amy Hin Yan, Chan, Katie, Van Leeuwen, Jolanda, Seetharaman, Ashwin, Aregger, Michael, Chandrashekhar, Megha, Hustedt, Nicole, Seth, Sahil, Noonan, Avery, Habsid, Andrea, Sizova, Olga, Nedyalkova, Lyudmila, Climie, Ryan, Tworzyanski, Leanne, Lawson, Keith, Sartori, Maria Augusta, Alibeh, Sabriyeh, Tieu, David, Masud, Sanna, Mero, Patricia, Weiss, Alexander, Brown, Kevin R., Usaj, Matej, Billmann, Maximilian, Rahman, Mahfuzur, Costanzo, Michael, Myers, Chad L., Andrews, Brenda J., Boone, Charles, Durocher, Daniel, Moffat, Jason
Формат: Artigo
Язык:Inglês
Опубликовано: Genetics Society of America 2017
Предметы:
Investigations
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5555476/
https://ncbi.nlm.nih.gov/pubmed/28655737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.117.041277
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