Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens

The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used to systematically create gene knockouts in a varie...

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Vydáno v:G3 (Bethesda)
Hlavní autoři: Hart, Traver, Tong, Amy Hin Yan, Chan, Katie, Van Leeuwen, Jolanda, Seetharaman, Ashwin, Aregger, Michael, Chandrashekhar, Megha, Hustedt, Nicole, Seth, Sahil, Noonan, Avery, Habsid, Andrea, Sizova, Olga, Nedyalkova, Lyudmila, Climie, Ryan, Tworzyanski, Leanne, Lawson, Keith, Sartori, Maria Augusta, Alibeh, Sabriyeh, Tieu, David, Masud, Sanna, Mero, Patricia, Weiss, Alexander, Brown, Kevin R., Usaj, Matej, Billmann, Maximilian, Rahman, Mahfuzur, Costanzo, Michael, Myers, Chad L., Andrews, Brenda J., Boone, Charles, Durocher, Daniel, Moffat, Jason
Médium: Artigo
Jazyk:Inglês
Vydáno: Genetics Society of America 2017
Témata:
Investigations
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5555476/
https://ncbi.nlm.nih.gov/pubmed/28655737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.117.041277
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