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Engineering Caspase 7 as an Affinity Reagent to Capture Proteolytic Products

Many proteases recognize their substrates with high specificities, with this in mind, it should theoretically be possible to utilize the substrate binding cleft of a protease as a scaffold to engineer an affinity reagent. In this study, we sought to develop reagents that would differentiate between...

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Publicat a:FEBS J
Autors principals: Razai, Amir S, Snipas, Scott J, Poreba, Marcin, Fasci, Domenico, Salvesen, Guy S
Format: Artigo
Idioma:Inglês
Publicat: 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7779697/
https://ncbi.nlm.nih.gov/pubmed/32619291
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/febs.15467
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