CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish

Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRN...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Amorim, João Pedro, Bordeira-Carriço, Renata, Gali-Macedo, Ana, Perrod, Chiara, Bessa, José
Format: Artigo
Language:Inglês
Published: Elsevier 2020
Subjects:
Protocol
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757680/
https://ncbi.nlm.nih.gov/pubmed/33377102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100208
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