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CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRN...
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| Veröffentlicht in: | STAR Protoc |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Elsevier
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757680/ https://ncbi.nlm.nih.gov/pubmed/33377102 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100208 |
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