Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish

BACKGROUND: The CRISPR/Cas9 system is a prokaryotic immune system that infers resistance to foreign genetic material and is a sort of 'adaptive immunity'. It has been adapted to enable high throughput genome editing and has revolutionised the generation of targeted mutations. RESULTS: We h...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Brocal, Isabel, White, Richard J., Dooley, Christopher M., Carruthers, Samantha N., Clark, Richard, Hall, Amanda, Busch-Nentwich, Elisabeth M., Stemple, Derek L., Kettleborough, Ross N. W.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4806435/
https://ncbi.nlm.nih.gov/pubmed/27009152
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-016-2563-z
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