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CRISPR/Cas9 cleavages in budding yeast reveal templated insertions and strand-specific insertion/deletion profiles

Harnessing CRISPR-Cas9 technology provides an unprecedented ability to modify genomic loci via DNA double-strand break (DSB) induction and repair. We analyzed nonhomologous end-joining (NHEJ) repair induced by Cas9 in budding yeast and found that the orientation of binding of Cas9 and its guide RNA...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Lemos, Brenda R., Kaplan, Adam C., Bae, Ji Eun, Ferrazzoli, Alexander E., Kuo, James, Anand, Ranjith P., Waterman, David P., Haber, James E.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5834694/
https://ncbi.nlm.nih.gov/pubmed/29440496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1716855115
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