Organoid Sample Preparation and Extraction for LC-MS Peptidomics

This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA, limiting the peptides that can be studied. However, an LC-MS-based appr...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Miedzybrodzka, Emily L., Foreman, Rachel E., Galvin, Sam G., Larraufie, Pierre, George, Amy L., Goldspink, Deborah A., Reimann, Frank, Gribble, Fiona M., Kay, Richard G.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757358/
https://ncbi.nlm.nih.gov/pubmed/33377058
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100164
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados