Organoid Sample Preparation and Extraction for LC-MS Peptidomics

This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA, limiting the peptides that can be studied. However, an LC-MS-based appr...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Miedzybrodzka, Emily L., Foreman, Rachel E., Galvin, Sam G., Larraufie, Pierre, George, Amy L., Goldspink, Deborah A., Reimann, Frank, Gribble, Fiona M., Kay, Richard G.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757358/
https://ncbi.nlm.nih.gov/pubmed/33377058
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100164
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