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TARGET-Seq: A Protocol for High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing

Single-cell RNA-sequencing technologies are ideally placed to resolve intratumoral heterogeneity. However, the lack of coverage across key mutation hotspots has precluded the correlation of genetic and transcriptional readouts from the same single cell. To overcome this, we developed TARGET-seq, a p...

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Publicat a:STAR Protoc
Autors principals: Rodriguez-Meira, Alba, O’Sullivan, Jennifer, Rahman, Haseeb, Mead, Adam J.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757016/
https://ncbi.nlm.nih.gov/pubmed/33377019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100125
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